Calls for the PT in 2022 (March and September)

ICAR is pleased to announce the organization of the ICAR Proficiency Test 2022.The participation in ICAR proficiency test will comply with analytical quality assurance requirements ISO 17025 and with the system of Certification of Quality implemented by ICAR from 2006 includes a recognition of the competence of laboratories and, more widely, of analytical systems in ICAR member organisation that requires regular PT participation with international anchorage. ICAR PT is ISO 17043 certified.


1.1 Administrative fees

The administrative fees are differentiated for ICAR and NO-ICAR members.If your laboratory is servicing an ICAR member you have to consider the member fee.For this reason in the subscription form we are asking you to report the organization’s name that you are providing the DHI results.

1.2 Parameter Fees

The technical sub contractor of this proficiency testing scheme is ACTALIA who has for many years collaborated efficiently with ICAR.The ICAR PT parameters considered are: fat, protein, urea, lactose, somatic cell, beta-Hydroxybutyrate (BHB), Pregnancy Associated Glycoproteins (PAG) and Microorganism DNA PCR technique in cow milk.For fat, protein, urea, lactose, somatic cell, beta-Hydroxybutyrate (BHB), it will be possible to participate with chemical (direct) method and alternative (indirect) method.ICAR secretariat will collect the participation forms, will send you the final report and the invoice.ICAR PT and ACTALIA PT will be organized in parallel. If you are interested in other parameters offered by Actalia you have to subscribe the Actalia order.The contract between ICAR and ACTALIA prescribes that for the ICAR PT parameters you will receive only that invoice from ICAR. ACTALIA will invoice your company only for the parameters that are NOT included in ICAR PT (extra-parameters)So to be clear, ICAR will invoice for the ICAR PT parameters and Actalia will invoice separately their extra parameters.We believe that the ICAR PT will be more useful for your quality assurance !A feature of the new ICAR PT report will help you track and trend your performance over time. ICAR technical staff will be there to help to interpret and support you as necessary.

2.0 Technical Information

Method to apply


Number of Samples

All methods chemical and alternative

Fat & Protein

10 samples with a volume of 65 ml preserved with 0.02 % bronopol, regularly ranging from 1.5 % to 4.9 % fat and from 2.5 % to 4.0 % crude protein (TNx6.38)

3 Kjeldahl solutions 1 tryptophan + 1 Glycin + 1 ammonium sulphate at N concentration of milk, with a volume of 30ml


10 samples with a volume of 65 ml preserved with 0.02 % bronopol, regularly ranging from 4.6 % to 5.1 % lactose

1 lactose solution with a volume of 30 ml at the concentration of milk

Somatic Cell

10 samples with a volume of 30 ml preserved with 0.02 % bronopol, regularly ranging from 50 to 1600 x103 cells/ml

2 lyophilized samples


10 samples with a volume of 30 ml preserved with 0.02 % bronopol, regularly ranging from 10 to 70 mg urea /100 ml


10 samples with a volume of 30 ml preserved with 0.02 % bronopol, regularly ranging from 0,06-0,25 mmole/liter


5 preserved samples with a volume of 12 ml for a qualitative determination positive and negative

DNA (PCR technique)

5 preserved samples with a volume of 2 ml for a qualitative determination positive and negative

Packaging: 65 ml , 30.12 and 2  ml polyethylene screw-capped vials with airtight joints, 2 glass vials for lyophilized milk.

Dispatch: by express carrier with ice in isolated boxes => arrival within 1 day (for most European countries) or up to 5 days, depending on the consignee’s country.

Storage in the laboratory: samples must be stored at 4°C upon arrival and should be analysed for SCC no longer than 5 days, and for chemistry no longer than 10 days, after the dispatch date.

Dates of sample dispatch

1st trial

7 March 2022

Arrival expected

from 8 to 10 March 2022

2nd trial

5 September 2022

Arrival expected

from 6 to 8 September 2022

3.0 Particular cautions for international delivery

Large delays can result from missing the exact fulfilment of the custom requirements of the importing country so it is important to update Actalia-Cecalait on the local custom requirements and the latest changes.

For new participants, particular attention should be paid to the first trial to check transport effectiveness and, where needed, enable possible improvement with regard to delays. The most appropriate method for sample transport and delivery will be determined.

Samples may arrive on a bank holiday in your country: please, be careful

4.0 ICAR PT Fees

The ICAR Administration fee is for each round
Shipping costs see the table below
Fees for each parameter and method are for each dispatch and for one set of samples


ICAR PT Fees –  Administrative fees

ICAR Member

150,00 €

No ICAR Member

200,00 €

Shipping cost/package

EU countries 50,00 €

No EU countries 80,00 €

Asia 180,00€

North Africa 100,00 €

Africa 150,00

North America 100,00

South America 250,00

Parameter Fees


Chemical (direct)

Alternative (indirect)


60,00 €

60,00 €


72,00 €

72,00 €


60,00 €

60,00 €


60,00 €

60,00 €

Somatic cells

98,00 €

98,00 €


97,00 €

97,00 €


63,00 €

DNA (PCR technique)

84,00 €

These prices do not include VAT


5.0 ICAR PT Subscription and payment

In case you do not have an ICAR PT account please register here

The already registered laboratories are requested to click here and fill in the following subscription form (Excel file)

The subscription has to be completed two weeks before the sample shipment that is scheduled on:

  • 7 March 2022
  • 5  September 2022

ICAR will invoce your laboratory; it has to be paid according to the deadline contained in the invoce.

Instructions on the use of the PT platform can be downloaded here

6.0 Results

Please upload your results to the ICAR PT platform. From this year onward, Excel file is no more accepted.

7. 0 Statistical treatment

The statistical treatment will be done according the ISO 17043 for each parameter.

The ICAR report scheme has been simplified by the ICAR Milk Analyses Sub-Committee and includes a full overview/explanation of the ICAR population.

All the data will be treated anonymously and a tight confidentiality commitment is guaranteed.

ICAR Secretariat will communicate you as soon as reports are available on ICAR PT platform

8.0 Contacts

For any further clarification, please do not hesitate to contact ICAR secretariat:

Silvia Orlandini email

Cesare Mosconi email

ICAR, Arthur van Schendelstraat 650, 3511 MJ Utrecht, The Netherlands - Email: