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Abstract by Juan Antonio Fernández - Merging of spectral datasets from different MIR instruments used in the routine analysis of milk

This work is performed in the framework of the OPTIMIR project. It aims to develop innovative farm management web applications based on the use of infrared spectroscopy analysis of milk to enable a sustainable and profitable management of the milk production. Mid Infrared (MIR) spectroscopy has been the method of choice worldwide for quality control during routine milk testing. It allows a fast, non destructive and screening quantification of milk chemical properties, as the content of fat, protein and lactose. In this project, the MIR spectrum will be used with a different purpose and will be considered as a reflexion of cows’ state in order to obtain indicators concerning fertility, health and feeding among others. This innovative approach of using MIR needs the support of important spectral databases associated to reference values for each of the properties to be studied. For this reason, a large number of commercially available MIR spectrometers from different manufacturers installed in different laboratories from four different countries were used. Because of differences of the instrumental responses between different MIR spectrometers, spectra obtained on one instrument cannot readily be compared to a library acquired on a different instrument. Moreover, the use of calibration models developed on an instrument with MIR spectra obtained on another instrument will usually lead to an increased uncertainty of the prediction model. Then, spectral corrections adapted to each instrument (standardization) are needed. In this work, the piecewise direct standardization (PDS) has been used in order to reduce the inherent instrument to instrument variability. The obtained results have shown a very good correlation between all the spectra and repeatable results across instruments, which is an indication that a common database could be constructed and would permit to develop MIR breeding tools which should be easily implemented in practice in many different countries.
 

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